| Primer | Species | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 11 | Ligilactobacillus ruminis | TGACACGGCAATGGTGGA | CAACAAGCGTTCAACGGCA | 59.17 | 59.94 | 214 | 138 |
100.00%
|
50.00%
|
| Primer 12 | Ligilactobacillus ruminis | TGACACGGCAATGGTGGA | CAAAACGTCGCATGGAGCC | 59.17 | 60.15 | 265 | 138 |
100.00%
|
50.00%
|
| Primer 13 | Ligilactobacillus ruminis | AGCAATTCTCAGGCGTCCC | TCGCCATATACGAACGGCA | 60.08 | 59.27 | 219 | 138 |
100.00%
|
50.00%
|
| Primer 14 | Ligilactobacillus ruminis | CAATGCGTCCGCTTTGCA | TTGCCGAGCGTTTGGTTG | 59.75 | 59.28 | 204 | 138 |
100.00%
|
50.00%
|
| Primer 15 | Ligilactobacillus ruminis | GCAATCCGACTTGTGGGGA | ACTGTGCAACGCCTTCCA | 60.00 | 59.81 | 264 | 135 |
100.00%
|
50.00%
|
| Primer 16 | Ligilactobacillus ruminis | TGCGCAATCCGACTTGTG | ACTGTGCAACGCCTTCCA | 58.75 | 59.81 | 267 | 135 |
100.00%
|
50.00%
|
| Primer 17 | Ligilactobacillus ruminis | CGATTTCCCAAGCTTCGGC | ACTGTGCAACGCCTTCCA | 59.57 | 59.81 | 174 | 135 |
100.00%
|
50.00%
|
| Primer 18 | Ligilactobacillus ruminis | CCCAAGCTTCGGCATCGAT | ACTGTGCAACGCCTTCCA | 60.52 | 59.81 | 168 | 135 |
100.00%
|
50.00%
|
| Primer 19 | Ligilactobacillus ruminis | TTGCGCAATCCGACTTGTG | ACTGTGCAACGCCTTCCA | 59.43 | 59.81 | 268 | 135 |
100.00%
|
50.00%
|
| Primer 20 | Ligilactobacillus ruminis | CCAAGCTTCGGCATCGATG | ACTGTGCAACGCCTTCCA | 59.35 | 59.81 | 167 | 135 |
100.00%
|
50.00%
|